论文部分内容阅读
作者建立了用自动生化分析仪测定血清苯巴比妥浓度的方法，并对此方法进行了评价。该测定方法的批内不精密度为5．6％～6．0％；批间不精密度为6．7％～7．6％；测定线性范围为0～80mg／L；标准曲线一次定标后，至少可使用一个月；将该法分别与荣光偏振免疫分析法（n=50）和高效液相色谱法（n=28）进行对比分析，相关系数分别为0．992和0．983；该法的平均回收率为98．6％；样本中的茶碱、苯妥英两种药物及轻度溶血、黄疸对测定结果无明显干扰。方法操作简便、精密度好，为广泛开展治疗药物监测工作创造了条件。
The authors established a method for determining the concentration of phenobarbital in serum using an automatic biochemical analyzer and evaluated the method. The intra-assay inaccuracy of the assay was 5.6% -6.0%; the inter-assay imprecision was 6.7% -7.6%; the linear range was 0-80 mg / L; the standard curve was determined once The standard method was used for at least one month. The results of the method were compared with the methods of polarization immunoassay (n = 50) and high performance liquid chromatography (n = 28) respectively, the correlation coefficients were 0.992 and 0.983 ; The average recovery rate of the method was 98.6%; theophylline, phenytoin in the sample and mild hemolysis, jaundice had no significant interference with the determination results. The method is easy to operate and has good precision, which has created conditions for the extensive monitoring of therapeutic drugs.