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免疫细胞化学结合图像分析研究表明，不同时辰电针大鼠“足三里”对交叉上核血管活性肠肽（ＶＩＰ）免疫反应性的影响效应不同。在白天光照条件下针刺，交叉上核中ＶＩＰ免疫反应性显著增加；而于无光照夜间针刺，交叉上核中ＶＩＰ免疫反应性呈现下降趋势。这种不同时辰针刺对交叉上核ＶＩＰ影响的效应不同与交叉上核内ＶＩＰ含量的变化有着密切关系。本研究结果提示不同时辰针刺“足三里”对机体神经内分泌活动产生不同影响。
Immunocytochemistry combined with image analysis showed that the effect of “Zusanli” electroacupuncture at different time points on the immunoreactivity of VIP on the cross was different. During daylight illumination, acupuncture at VIP significantly increased VIP immunoreactivity in the suprachiasmatic nucleus; while in the absence of light acupuncture at night, VIP immunoreactivity in the suprachiasmatic nucleus showed a downward trend. The effect of different time acupuncture on the effects of VIP on the suprachiasmatic nucleus is closely related to the changes of VIP content in the nucleus on the cross. The results suggest that different time acupuncture “Zusanli” on the body neuroendocrine activity have different effects.