论文部分内容阅读
目的采用改良后的基于流式细胞术的大鼠体内Pig-a基因突变试验方法来检测和评价亚硒酸钠的体内致突变作用,为研究亚硒酸钠的遗传毒性和致癌性提供一定的科学依据。方法将30只雄性SD大鼠分别连续3天灌胃给予0.625(低)、1.25(中)和2.5 mg/kg.bw(高)亚硒酸钠,同时连续3天给予40 mg/kg.bw N-乙基-N-亚硝基脲(ENU)作为阳性对照。在给药前和首次给药后15天、30天和45天进行尾静脉采血,利用流式细胞仪检测样本中红细胞突变率。结果随着染毒时间的延长及染毒剂量增大,RBCCD59-率和RETCD59-率均呈上升趋势。15天时,1.25 mg/kg.bw组RBCCD59-率高于空白对照组,差异有统计学意义(P<0.05),30天时,1.25、2.5 mg/kg.bw组RBCCD59-率高于空白对照组,差异有统计学意义(P<0.05)。45天时,亚硒酸钠各组RBCCD59-率均高于空白对照组,差异有统计学意义(P<0.05);30天和45天时,亚硒酸钠高剂量组RETCD59-率高于空白对照组,差异有统计学意义(P<0.05)。同时随时间延长,出现大鼠体重下降的趋势,且中、高剂量组均出现了死亡的现象。结论亚硒酸钠在一定剂量下具有弱致突变作用,同时还具有一般毒性作用。
Objective To detect and evaluate the in vivo mutagenicity of sodium selenite by using the modified Pig-a gene mutation assay in rats based on flow cytometry, and to provide some evidence for studying the genetic toxicity and carcinogenicity of sodium selenite Scientific basis. Methods Thirty male Sprague-Dawley rats were orally administered 0.625 (low), 1.25 (medium) and 2.5 mg / kg.bw (high) sodium selenite orally for 3 consecutive days respectively, while given 40 mg / kg.bw N-ethyl-N-nitrosourea (ENU) as a positive control. Blood samples were taken from the tail vein before and 15 days, 30 days and 45 days after the first administration, and the mutation rate of erythrocytes in the samples was detected by flow cytometry. Results With the prolongation of exposure time and the increase of exposure dose, the rates of RBCCD59 and RETCD59-R showed an upward trend. At 15 days, the rate of RBCCD59 in the 1.25 mg / kg.bw group was higher than that in the blank control group (P <0.05). At 30 days, the rates of RBCCD59 in the 1.25 and 2.5 mg / kg.bw groups were higher than those in the blank control group , The difference was statistically significant (P <0.05). On the 45th day, the RBCCD59-rates of sodium selenite groups were higher than those of the blank control group (P <0.05). At 30 days and 45 days, the RETCD59-rates of sodium selenite high-dose group were higher than those of the blank control Group, the difference was statistically significant (P <0.05). At the same time, the body weight of the rats tended to decrease with the passage of time, and the death occurred in the medium and high dose groups. Conclusion Sodium selenite has a weak mutagenic effect at a certain dose, but it also has general toxicity.