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我们利用AdEasy系统在细菌内构建携带非分泌型尿激酶型纤溶酶原激活剂（urokinase-type plasminogen acti-vator,uPA）和绿色荧光蛋白（greenfluorescent protein,GFP）cDNA的复制缺陷型腺病毒,并观察其在肝星形细胞（hepatic stellate cell,HSC）中的表达。
We used the AdEasy system to construct replication-defective adenovirus carrying non-secreted urokinase-type plasminogen acti-vator (uPA) and greenfluorescent protein (GFP) cDNA in bacteria. And observe its expression in hepatic stellate cells (HSC).