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用基因交换的方法将烟草花叶病毒普通株（中国分离物，ＴＭＶ－Ｃｖ和番茄花叶病毒弱毒疫苗Ｎ１４基因组的运动蛋白（ＭＰ）、外壳蛋白（ＣＰ）基因编码区和３＇非编码区嵌合于Ｎ１４和ＴＭＶ－ＣＶ ５＇非编码区和复制酶基因的下游，构建重组病毒克隆ｐＴＮ和ｐＮＴ，并进行体外转录和烟草侵染试验结果 ｐＴＮ和 ｐＮＴ体外转录物对烟草均具侵染性：前者感染枯斑三生烟（ Ｎｉｃｏｔｉａｎａ ｔｏｂａｃｕｍ ｃｖ．Ｓａｍｓｕｎ ＮＮ），等量感染的枯斑形成数比 ＰＴＭＶ－Ｃｖ少；感染普通烟（ Ｎｉｃｏｔｉａｎａ ｔｏｂａｃｕｍ ｃｖ． Ｈｕａｎｇｍ－ｉａｏｙｕ，黄苗榆品种），出现典型系统症状，但叶片畸形率较低．后者感染枯斑三生烟，等量感染的枯斑形成数比ｐＮ１４多；感染普通烟仍不出现系统症状．结果表明：重组病毒ＴＮ和ＮＴ仍能在试验用烟草中增殖，前者在普通烟上基本表现为ＴＭＶ－Ｃｖ的强病毒性状，后者在普通烟上表现为Ｎ１４的弱病毒性状； ＴＭＶ－ＣＶ和 Ｎ１４的复制酶与 ＭＰ， ＣＰ和 ３＇非编码区之间为非严紧型连接，两者在类似的各亚基因组之间可进行功能互补．初步推测，Ｎ１４复制酶编码基因的突变区对Ｎ１４的致弱起主要作用；其运动蛋白编码基因的突变区对被侵染烟草
(MP), coat protein (CP) gene coding region and 3 ’non-coding region of tobacco mosaic virus common strains (Chinese isolate, TMV-Cv and tomato mosaic virus attenuated vaccine N14 genomes by gene exchange method) Chimeric downstream of the N14 and TMV-CV 5 ’non-coding regions and the replicase gene, the recombinant virus clones pTN and pNT were constructed and subjected to in vitro transcription and tobacco infestation test results. In vitro transcripts of pTN and pNT were both infective to tobacco The former was infected with Nicotiana tobacum cv.Samsun NN and the number of necrotic lesions was less than that of PTMV-Cv. The infection of Nicotiana tobacum cv. Huangm-iaoyu But the rate of leaf deformity was lower, the latter was infected with dead spot and the number of necrosis was more than that of pN14.While infected with normal tobacco still showed no systemic symptoms, the results showed that: The viruses TN and NT could still proliferate in the experimental tobacco, the former basically showed the strong virulence of TMV-Cv on common tobacco, while the latter showed the weak virus character of N14 on common tobacco; the replication of TMV-CV and N14 Enzymes and MP, CP and 3 ’non-coding region between non-tight junction, both in similar sub-genomes can complement each other.It is presumed that the mutation of N14 replicase gene coding region of N14 weaken Played a major role; the mutation region of its coding genes for kinetic proteins was infected with tobacco