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目的:探讨胃癌中STAT3、Survivin和RegⅣ的表达及其与临床病理参数间的相关性,为临床治疗提供合理依据。方法:选取2013年1月至2015年1月本院采集的经病理学证实并接受手术切除治疗的70例胃癌组织石蜡标本,另取70例胃癌癌旁组织,配对30例正常胃组织;采用免疫组化染色法检测胃癌组织、癌旁组织以及正常胃黏膜组织标本中STAT3、Survivin及RegⅣ的表达水平,分析STAT3、Survivin及RegⅣ的表达与胃癌患者临床病理参数之间关系。结果:STAT3、Survivin及RegⅣ在胃癌组织中的阳性表达率明显高于胃癌旁组织和胃正常黏膜组织,差异均具有统计学意义(P<0.01)。STAT3、Survivin及RegⅣ在癌旁组织阳性表达率高于正常组织,但差异无统计学意义(P>0.05)。患者年龄、性别、肿瘤大小与胃癌组织中STAT3、Survivin及RegⅣ蛋白的表达均无关(P>0.05),而肿瘤细胞分化程度、浸润深度、淋巴结转移及肿瘤TNM分期与胃癌组织中STAT3、Survivin及RegⅣ蛋白的表达相关,差异均具有统计学意义(P<0.05)。当肿瘤细胞分化程度较低、浸润深度较深、伴有淋巴结转移、TNM分期较晚时,胃癌组织中的STAT3、Survivin及RegⅣ蛋白的表达阳性率较高(P<0.05)。Spearman等级相关性分析结果表明,STAT3、Survivin及RegⅣ呈正相关(P<0.01)。结论:肿瘤细胞分化程度较低、浸润较深、存在淋巴结转移及TNM分期较晚与胃癌组织中STAT3、Survivin及RegⅣ蛋白的表达呈正相关,且三者在胃癌组织中的表达均呈正相关,可能与胃癌的发生、侵袭、转移以及患者的不良预后存在密切联系。联合检测胃癌组织中三者表达情况有助于对胃癌恶性程度和患者的预后的临床判断并为基因靶向治疗提供理论依据。
Objective: To investigate the expression of STAT3, Survivin and Reg IV in gastric carcinoma and its correlation with clinicopathological parameters, and provide a reasonable basis for clinical treatment. METHODS: From January 2013 to January 2015, 70 specimens of gastric cancer tissues confirmed by pathology and surgically treated and collected from our hospital were selected, and another 70 specimens of adjacent gastric cancer tissues were taken and matched with 30 normal gastric tissues. Immunohistochemical staining was used to detect the expression of STAT3, Survivin and Reg IV in gastric cancer tissues, paracancerous tissues and normal gastric mucosa. The relationship between the expression of STAT3, Survivin and Reg IV and clinicopathological parameters of gastric cancer was analyzed. Results: The positive rates of STAT3, Survivin and Reg IV in gastric cancer tissues were significantly higher than those in para-gastric tissues and normal gastric mucosa tissues. The differences were statistically significant (P<0.01). The positive rate of STAT3, Survivin and Reg IV in the adjacent tissues was higher than that in normal tissues, but the difference was not statistically significant (P>0.05). There was no relationship between the age, gender, tumor size and the expression of STAT3, Survivin, and Reg IV protein in gastric cancer tissues (P>0.05). However, the tumor cell differentiation, depth of invasion, lymph node metastasis, tumor TNM staging, and STAT3, Survivin in gastric cancer tissues. The expression of RegIV protein was related, and the difference was statistically significant (P<0.05). When the tumor cells were less differentiated, deeper infiltration, lymph node metastasis, and late TNM staging, the positive rates of STAT3, Survivin, and RegIV proteins were higher in gastric cancer tissues (P<0.05). Spearman rank correlation analysis showed that STAT3, Survivin and RegIV were positively correlated (P<0.01). Conclusion: The low degree of differentiation, deep invasion, lymph node metastasis, and late TNM staging of tumor cells are positively correlated with the expression of STAT3, Survivin, and Reg IV proteins in gastric cancer tissues. The expression of these three genes in gastric cancer tissues is positively correlated. It is closely related to the occurrence, invasion, metastasis of gastric cancer and poor prognosis of patients. The combined detection of three expressions in gastric cancer can contribute to the clinical judgment of the malignancy of gastric cancer and the prognosis of patients and provide a theoretical basis for targeted gene therapy.