论文部分内容阅读
目的 评价大鼠同种异体子宫异位移植模型建立的手术学方法及其可行性。方法选择8~10周龄Wistar大鼠60只,分为供体30只和受体30只,行子宫异位移植手术。记录每例手术的时间(前15例手术为预实验组,后15例手术为实验组)。术后7d处死受体大鼠,评估移植子宫的组织形态学变化。结果 共进行同种异体子宫异位移植手术30例,存活率由预实验组的40% (6 /15)上升至实验组的75% (12 /15)。存活的18只受体大鼠中, 15只移植子宫成活,移植子宫成活率为83%。预实验组子宫移植的供体手术时间、受体手术时间、子宫移植时血管吻合时间和总手术时间分别为(68±8)min、(110±20)min、(39±5 )min和( 178±24 )min,实验组分别为( 65±10 )min、( 89±22)min、(36±8)min和(154±23)min,后者与前者比较,各步骤手术时间及总手术时间均缩短,但差异无统计学意义。结论 Wistar大鼠异体子宫异位移植模型的建立是可行的,并将为人类子宫移植提供实验依据。
Objective To evaluate the feasibility and feasibility of establishing a surgical model of allogeneic uterine allograft in rats. Methods Sixty Wistar rats aged 8 to 10 weeks were divided into 30 donors and 30 recipients undergoing uterine allotransplantation. The time of each operation was recorded (the first 15 cases were pre-experimental group and the last 15 cases were experimental group). Recipient rats were sacrificed on day 7 postoperatively to assess histomorphological changes in the transplanted uterus. Results A total of 30 cases of allogeneic uterine allograft were performed. The survival rate increased from 40% (6/15) in the pre-experimental group to 75% (12/15) in the experimental group. Of the 18 surviving 18 recipient rats, 15 survived the uterus and had a survival rate of 83%. The donor operation time, recipient operation time, vascular anastomosis time and total operation time in the pre-experimental group were (68 ± 8) min, (110 ± 20) min, (39 ± 5) min and 178 ± 24) min, and the experimental groups were (65 ± 10) min, (89 ± 22) min, (36 ± 8) min and (154 ± 23) min respectively. The operation time was shortened, but the difference was not statistically significant. Conclusion Wistar rat heterotopic uterine transplantation model is feasible and will provide experimental evidence for human uterine transplantation.