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目的为探讨WO3纳米方块对小鼠肝脏和肾脏组织的急性氧化损伤作用。方法将42只Balb/c小鼠随机分为7组,每组6只,分别为5、10、20、40、80、160 mg/kg的WO3纳米方块染毒组和生理盐水对照组。灌胃染毒7 d后测定肝脏和肾脏组织中活性氧(ROS)、还原性谷胱甘肽(GSH)以及丙二醛(MDA)的含量。结果随着WO3纳米方块染毒剂量的升高,肝脏和肾脏组织的ROS和MDA的含量均呈逐渐上升的趋势,而GSH含量呈逐渐降低的趋势,各指标均呈一定的剂量-效应关系。剂量≥20 mg/kg时肝脏组织ROS含量和剂量≥10 mg/kg时肾脏组织ROS含量分别与对照组比较,差异有统计学意义(P<0.05);剂量≥20 mg/kg时,肝脏和肾脏组织MDA含量与对照组比较,差异有统计学意义(P<0.05);剂量≥40 mg/kg时肝脏组织GSH含量和剂量≥20 mg/kg时肾脏组织GSH含量分别与对照组比较,差异有统计学意义(P<0.05)。结论一定剂量的WO3纳米方块能够对小鼠肝脏和肾脏造成急性氧化损伤。
Aim To investigate the effect of WO3 nanoblocks on acute oxidative damage in liver and kidney of mice. Methods Forty-two Balb / c mice were randomly divided into 7 groups (n = 6, n = 5, 10, 20, 40, 80 and 160 mg / kg) The levels of reactive oxygen species (ROS), reducing glutathione (GSH) and malondialdehyde (MDA) in liver and kidney tissues were measured after 7 days of intragastric administration. Results The contents of ROS and MDA in liver and kidney increased gradually with the increase of the dose of WO3 nanoparticles, while the content of GSH gradually decreased. All the indexes showed a dose-response relationship. The ROS content in liver tissue at the dose of 20 mg / kg and the ROS content in the kidney at the dose of 10 mg / kg were significantly different from those in the control group (P <0.05). At the dose of 20 mg / kg, Compared with the control group, the content of MDA in kidney tissue had statistical significance (P <0.05). The GSH content in liver tissue and the content of GSH in kidney tissue at the dose of 40 mg / kg were higher than those in the control group There was statistical significance (P <0.05). Conclusion A certain dose of WO3 nano-cube can cause acute oxidative damage to the liver and kidney of mice.