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目的探讨微小RNA-21(miR-21)在宫颈鳞癌中的表达及其与临床分期、病灶大小、分化程度、淋巴结转移、先期化疗的关系。方法收集2009年4月至2012年4月南华大学附属第一医院和附属南华医院50例宫颈鳞癌患者的临床资料。同时在病理科查找出相对应的石蜡包埋组织。手工显微切割宫颈鳞癌组织,实时荧光定量PCR检测miR-21的表达,对其表达水平与临床病理特征进行统计分析。结果宫颈鳞癌组织中平均miR-21相对表达水平明显高于正常组织,差异具有统计学意义(t=3.026,P<0.05)。宫颈鳞癌低分化组中miR-21相对表达量明显高于宫颈鳞癌高分化组,差异具有统计学意义(t=2.248,P<0.05)。宫颈鳞癌淋巴结转移组中miR-21相对表达量明显高于宫颈鳞癌无淋巴结转移组,差异具有统计学意义(t=2.532,P<0.05)。宫颈鳞癌FIGOⅠ期组和FIGOⅡ期组、宫颈鳞癌大病灶组和小病灶组、宫颈鳞癌先期化疗组和未先期化疗组miR-21相对表达量比较差异均无统计学意义。结论 miR-21在宫颈鳞癌组织中表达增高,与分化程度和淋巴结转移有关,与临床分期、癌灶大小、先期化疗关系不密切。
Objective To investigate the expression of microRNA-21 (miR-21) in cervical squamous cell carcinoma and its relationship with clinical stage, size, differentiation, lymph node metastasis and advanced chemotherapy. Methods The clinical data of 50 patients with cervical squamous cell carcinoma from the First Affiliated Hospital of Nanhua University and Affiliated Nanhua Hospital from April 2009 to April 2012 were collected. At the same time find out in the pathology of the corresponding paraffin-embedded tissue. The cervical squamous cell carcinoma tissue was microdissected by hand, the expression of miR-21 was detected by real-time fluorescence quantitative PCR, and the expression level and clinicopathological features were analyzed statistically. Results The average relative expression of miR-21 in cervical squamous cell carcinoma tissues was significantly higher than that in normal tissues (t = 3.026, P <0.05). The relative expression of miR-21 in cervical squamous cell carcinoma was significantly higher than that in cervical squamous cell carcinoma (t = 2.248, P <0.05). The relative expression of miR-21 in cervical squamous cell carcinoma with lymph node metastasis was significantly higher than that in cervical squamous cell carcinoma without lymph node metastasis (t = 2.532, P <0.05). Cervical squamous cell carcinoma of FIGO Ⅰ group and FIGO Ⅱ group, cervical squamous cell carcinoma of large lesions group and small lesions group, cervical squamous cell carcinoma of the early chemotherapy group and no pre-chemotherapy group relative expression of miR-21 relative differences were not statistically significant. Conclusion The expression of miR-21 is increased in cervical squamous cell carcinoma, which is related to the degree of differentiation and lymph node metastasis. It is not closely related to the clinical stage, the size of tumor and the advanced chemotherapy.