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目的:建立测定骆驼刺中总还原糖与芦丁含量的方法,并对不同产地不同月份的样品含量进行比较。方法:采用紫外分光光度法测定骆驼刺中总还原糖的含量,以葡萄糖为对照品,检测波长为620nm;采用高效液相色谱法测定芦丁的含量,色谱柱为SymmetryShieldRp-18(150mm×3.9mm,5μm),流动相为乙腈-0.1%磷酸水溶液(19∶81,V/V),检测波长为360nm,柱温为30℃。结果:葡萄糖检测浓度的线性范围为3.85～70.33μg·mL-(1r=0.9959),平均加样回收率为99.6%,RSD=1.5%(n=6);芦丁检测浓度的线性范围为4.95～59.40μg·mL-(1r=0.9996),平均加样回收率为99.3%,RSD=2.08%(n=6)。不同产地不同月份的骆驼刺中芦丁和总还原糖含量有一定差异,其中新疆乌鲁木齐和且末地区在7-8月份采收的骆驼刺中总还原糖和芦丁的含量较高。结论:本试验结果可为骆驼刺的种植和采收的研究奠定基础。
Objective: To establish a method for determination of total reducing sugars and rutin in Alhagi sparsifolia, and to compare the contents of different samples in different months. Methods: The content of total reducing sugars in Alhagi sparsifolia was determined by UV spectrophotometry. The detection wavelength was 620 nm with glucose as reference substance. The content of rutin was determined by HPLC. SymmetryShieldRp-18 (150 mm × 3.9 mm, 5μm). The mobile phase was acetonitrile-0.1% phosphoric acid solution (19:81, V / V). The detection wavelength was 360nm and the column temperature was 30 ℃. Results: The linear range of detection concentration of glucose was 3.85-70.33μg · mL- (1r = 0.9959). The average recovery was 99.6%, RSD was 1.5% (n = 6). The linear range of detection concentration of rutin was 4.95 ~ 59.40μg · mL- (1r = 0.9996), the average recovery was 99.3%, RSD = 2.08% (n = 6). The contents of rutin and total reducing sugars in camel stings in different months were different. Among them, the content of total reducing sugars and rutin in camel’s thorn collected from July to August in Urumqi, Xinjiang was higher. Conclusion: The results of this experiment can lay the foundation for the research of planting and harvesting of Alhagi sparsifolia.