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目的探讨IL-11对4.0 Gy中子辐射小鼠肠道IL-11受体表达影响。方法136只BALB/C二级小鼠,经4.0Gy中子全身照射,于照前3 d或照后即刻注射600μg/kg rhIL-11,每日1次,连用3 d,并于照后6 h、1 d、2 d、5 d活杀取小肠,采用SP免疫组织化学和图像分析等技术,定量研究肠组织中IL-11、IL-11Rα和gp130表达变化。结果正常小鼠中,IL-11和gp130于肠绒毛上皮细胞和隐窝细胞浆呈强阳性,尤以绒毛顶端细胞为显著;IL-11Rα于肠绒毛全层上皮细胞和隐窝细胞浆呈强阳性,其强度高于IL-11。4.0 Gy中子照后2 d内,IL-11、IL-11Rα和gp130于肠绒毛和隐窝上皮细胞表达均明显减少;照前和照后应用IL-11,三者阳性强度均高于4.0 Gy照射组。结论4.0 Gy中子照射后,小鼠肠道IL-11、IL-11Rα和gp130表达减少,应用IL-11刺激肠上皮细胞IL-11受体表达上调,且该作用参与了中子辐射后肠道损伤与修复的病理生理过程。
Objective To investigate the effect of IL-11 on the expression of IL-11 receptor in mice with 4.0 Gy neutron radiation. Methods Thirty-six BALB / c mice were irradiated with 4.0 Gy neutron whole body and injected with 600 μg / kg rhIL-11 three days before or immediately after irradiation for 3 days. The small intestine was killed on day 1, day 2, day 2, day 5, and SP immunohistochemistry and image analysis were used to quantitatively study the expression of IL-11, IL-11Rα and gp130 in intestinal tissue. Results In normal mice, IL-11 and gp130 were strongly positive in the villus villus epithelial cells and crypts, especially in the villus apical cells. IL-11Rα was found to be strongly expressed in the intestinal epithelial cells and crypts IL-11, IL-11Rα and gp130 were significantly decreased in the villus and crypt epithelial cells within 2 days after neutron irradiation with IL-11.4.0 Gy, 11, the three positive intensity were higher than the 4.0 Gy irradiation group. Conclusion The expression of IL-11, IL-11Rα and gp130 in intestinal tract of mice decreased after irradiation with 4.0 Gy neutron. The IL-11 receptor was up-regulated in intestinal epithelial cells stimulated with IL-11, Road injury and repair of the pathophysiological process.