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Objective To investigate the effect of n-butanol extract from Potentilla anserina(NP)intervention on hypoxia-induced Ca 2+ overload and SERCA2 expression of rat cardiomyocytes.Methods Primary cultured myocardial cell from SD neonatal rat(1-3 d)was used in the establishment of hypoxia model.After hypoxia for 3 h,the Ca 2+ concentration of myocardial cells was measured with fura-2/AM fluorescent probe,and the biochemical indicator intracellular Ca 2+ -ATPase was examined and the mRNA and its protective protein levels of the sarcoplasmic reticulum(SR)Ca 2+ -ATPases(SERCA2)were assayed with RT-PCR,Western-blotting,and immune-cytochemical staining in each group.Results The results showed that NP decreased Ca 2+ concentration, increased the activity of Ca 2+ -ATPase,and improved the mRNA and protein expression of SERCA2 in hypoxia-injured myocardial cells as compared with the model group.Conclusion These results indicate that NP could attenuate the Ca 2+ overload.The mechanism might be explained as that NP could elevate the SERCA2 level, increase the activity of myocardium in rats,and further enhance the capacity of SR Ca 2+ re-uptake.
Objective To investigate the effect of n-butanol extract from Potentilla anserina (NP) intervention on hypoxia-induced Ca 2+ overload and SERCA2 expression of rat cardiomyocytes. Methods Primary cultured myocardial cells from SD neonatal rat (1-3 d) was used in the establishment of hypoxia model. After hypoxia for 3 h, the Ca 2+ concentration of myocardial cells was measured with fura-2 / AM fluorescent probe, and the biochemical indicator intracellular Ca 2+ -ATPase was examined and the mRNA and its protective protein levels of the sarcoplasmic reticulum (SR) Ca 2+ -ATPases (SERCA2) were assayed with RT-PCR, Western-blotting, and immune-cytochemical staining in each group. Results Results The results showed that NP decreased Ca 2+ concentration, increased the activity of Ca 2+ -ATPase, and improved the mRNA and protein expression of SERCA2 in hypoxia-injured myocardial cells as compared with the model group. Conclusions These results indicates that NP could attenuate the Ca 2+ overload. The mechanism might be explant ined as that NP could elevate the SERCA2 level, increase the activity of myocardium in rats, and further enhance the capacity of SR Ca 2+ re-uptake.