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根据已知的日本血吸虫菲律宾株的副肌球蛋白分子的部分ｃＤＮＡ序列，设计两对寡核苷酸引物（引物１／２和引物３／４），以聚合酶链式反应（ＰＣＲ），用引物１／２从本室两个日本血吸虫中国大陆株ｃＤＮＡ库中均扩增出与预期大小（９２７ｂｐ）一致的特定ＤＮＡ片段。巢式ＰＣＲ——以第一扩增产物为模板，用引物３／４扩增出约５００ｂｐ的单一条带，与预期片段（４９４ｂｐ）大小一致。表明ＰＣＲ产物为编码副肌球蛋白的目的基因片段。
Two pairs of oligonucleotide primers (Primer 1/2 and Primer 3/4) were designed based on the partial cDNA sequence of the Paramyosin molecule of the known S. japonicum Philippine strain, using polymerase chain reaction (PCR) Primer 1/2 A DNA fragment of the same size as the expected size (927 bp) was amplified from cDNA libraries of two Chinese Schistosoma japonicum strains in our laboratory. Nested PCR - Using the first amplification product as a template, a single band of about 500 bp was amplified with primer 3/4, which was the same size as the expected fragment (494 bp). This indicates that the PCR product is a target gene fragment encoding paramyosin.