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Light dose plays an important role in the non-ablative photorejuvenation based on the theory of selective photothermolysis. However, present techniques for determining the light dose in clinical practice are still not accurate enough. We present a new system which monitors the skin tissue response during the laser irradiation in photorejuvenation by measuring the change of the total attenuation coefficient. We also investigate the relationship between the total attenuation coefficient and the energy density, pulse duration, and pulse repetition. In this procedure, the total attenuation coefficient decreases when the light dose increases and the reduction dependes on the light dose. These experimental results indicate that the new system would be a potential tool for accurate light dose determination.