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目的探讨核转录因子激活蛋白2α(AP-2α)对A549细胞环氧合酶2(COX-2)表达的作用。方法将AP-2α腺病毒载体转染入A549细胞,观察AP-2α对A549 细胞COX-2 表达及白介素1β(IL-1β)诱导A549细胞 COX-2表达的影响。将AP-2突变质粒载体转染入A549细胞,观察AP-2突变体对IL-1β诱导A549细胞 COX-2表达的影响。用Western印迹检测COX-2蛋白的表达。结果AP-2α增加 A549 细胞 COX-2表达及IL-1β诱导的A549细胞COX-2表达。AP-2突变体降低IL-1β诱导的A549细胞COX-2表达。结论AP-2α上调A549细胞COX-2的表达。
Objective To investigate the effect of AP-2α on cyclooxygenase-2 (COX-2) expression in A549 cells. Methods AP-2α adenovirus vector was transfected into A549 cells and the effect of AP-2α on COX-2 expression and COX-2 expression in A549 cells induced by interleukin-1β (IL-1β) was observed. The AP-2 mutant plasmid vector was transfected into A549 cells to observe the effect of AP-2 mutant on IL-1β-induced COX-2 expression in A549 cells. COX-2 protein expression was detected by Western blotting. Results AP-2α increased COX-2 expression in A549 cells and COX-2 expression in A549 cells induced by IL-1β. The AP-2 mutant reduced IL-1β-induced COX-2 expression in A549 cells. Conclusion AP-2α up-regulates the expression of COX-2 in A549 cells.