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为了实现对待测样品高质量的体视显微成像,提出了基于可编程发光二极管(LED)阵列照明的透射体视显微镜。该显微镜光学系统使用可编程LED阵列作为照明双光源,照明孔径、角度以及波长均可自由选择,通过改变可编程LED阵列上红蓝圆形光源的半径r和圆心间距d分别实现对体视显微镜焦深和体视角的灵活可控。实验结果表明,该显微镜系统简便,观察者根据自身需要选择合适的焦深和体视角可有效解决因人眼瞳距个体差异大引起单一体视角出现的双像不能重合等问题,实现对待测样品的直接体视显微观看。使用数值孔径NA=0.1(4×)的显微物镜,在最佳参数r=2,d=3时可清晰观测到待测样品的层次关系以及相对位置等三维信息。
In order to achieve high-quality stereoscopic imaging of the sample under test, a transmissive stereomicroscope based on a programmable light-emitting diode (LED) array illumination has been proposed. The microscope optical system uses a programmable LED array as the illumination dual light source, the illumination aperture, the angle and the wavelength are freely selectable. By changing the radius r and the center distance d of the red and blue circular light sources on the programmable LED array, Focal depth and body angle of the flexible and controllable. The experimental results show that the microscope system is simple, the observer can select the appropriate depth of focus and body viewing angle according to their own needs, which can effectively solve the problem that the double images caused by the large individual difference of the pupil of the human eye can not overlap. Therefore, Direct stereoscopic microscopic view. The microscopic objective lens with numerical aperture NA = 0.1 (4 ×) can clearly observe three-dimensional information such as the hierarchical relationship and the relative position of the sample to be measured under the optimum parameters of r = 2 and d = 3.