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目的探讨E-钙黏附素(E-cadherin)基因甲基化与结直肠癌临床病理特征的关系。方法采用甲基化特异性巢式PCR技术(nMSP法)研究100例结直肠癌组织及100例癌旁组织E-cadherin基因甲基化与临床病理特征的关系。结果100例结直肠癌组织中有33例呈E-cad基因甲基化阳性(阳性率33.0%),相应的癌旁组织无甲基化。Dukes分期中C、D期组织E-cad基因甲基化阳性率(72.7%)明显高于A、B期(27.3%)(P<0.05);伴肝转移的结直肠癌组织甲基化阳性率(81.8%)明显高于无肝转移者(18.2%)(P<0.01);在肿瘤细胞分化程度、大体类型、年龄、性别和肿瘤部位等其他病理特征,E-cad基因甲基化阳性组与阴性组差异无统计学意义(P>0.05)。结论结直肠癌组织E-cad基因甲基化与淋巴结转移、肝转移和Dukes分期明显相关,E-cad基因甲基化可能是结直肠癌侵袭性增强的原因之一。
Objective To investigate the relationship between methylation of E-cadherin gene and the clinicopathological features of colorectal cancer. Methods Methylation-specific nested PCR (nMSP) was used to investigate the relationship between methylation and clinicopathological features of E-cadherin gene in 100 cases of colorectal cancer tissues and 100 cases of paracancerous tissues. Results E-cadherin gene methylation was positive in 33 of 100 cases of colorectal cancer (positive rate was 33.0%), and the corresponding paracancerous tissues were not methylated. The positive rate of methylation of E-cadherin in stage C and D in Dukes stage was significantly higher than that in stage A and B (27.3%) (P <0.05). The positive methylation of E-cadherin in colorectal cancer tissues with liver metastasis (81.8%) were significantly higher than those without liver metastasis (18.2%) (P <0.01). The methylation of E-cad was positive in other pathological features such as tumor cell differentiation, general type, age, sex and tumor location No significant difference between the group and the negative group (P> 0.05). Conclusion The methylation of E-cad gene in colorectal cancer tissues is significantly associated with lymph node metastasis, liver metastasis and Dukes stage. Methylation of E-cad gene may be one of the reasons for the increased invasiveness of colorectal cancer.