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采用TTC法和过氧化物酶法测定花粉活力及寿命,用联苯胺-过氧化氢法测定柱头可授性,并对不同授粉方式的植株进行结实特征调查。结果表明:1金线兰与台湾金线莲在花粉活力上表现出相同的趋势,先上升后下降;贮藏温度与贮藏时间对金线莲花粉活力存在显著的影响,随着贮藏时间的延长,花粉活力降低,4℃冰箱贮藏可适当延长花粉活力。2柱头于开花当天就具有可授性,第4天最强,随后逐渐降低,金线兰于花后第8天不具可授性,而台湾金线莲于花后第10天不具可授性。3不同授粉方式对金线莲结实率影响显著,人工异花授粉的结实率远远高于人工自花授粉。因此,采集花后第3天花粉对花后第4天的柱头进行人工异花授粉杂交,可显著提高结实率,为金线莲新品种的选育和良种繁育提供技术保证。
Pollen viability and longevity were measured by TTC method and peroxidase method. The stigma receptivity was determined by benzidine - hydrogen peroxide method. The pollination characteristics of the pollination plants were investigated. The results showed that the pollen viability of A. japonicus and A. xylostella showed the same trend at first, then rose and then decreased. The storage temperature and storage time had significant effects on the pollen viability. With the extension of storage time, Pollen vitality decreased, 4 ℃ refrigerator storage may be appropriate to extend the vitality of pollen. 2 stigma was tolerant on the day of flowering, the strongest on day 4, then gradually decreased, the golden thread blue was not acceptable on the 8th day after flowering, while the golden thread on the 10th day after flowering was not acceptable . Different pollination methods had a significant effect on the seed setting rate of A. lotus, the seed setting rate of artificial cross-pollination was much higher than that of artificial self-pollination. Therefore, collecting pollen on the third day after anthesis made artificial cross-pollination cross on the stigma of the fourth day after anthesis, which could significantly improve the seed setting rate and provide technical assurance for the breeding and breeding of the new species.