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目的:探讨大连地区健康体检女性人乳头瘤病毒(HPV)的感染情况,为宫颈癌的筛查及防治提供理论依据。方法:采用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术对大连地区454例健康体检女性HPV感染进行定性检测并加以分型。结果:454例标本中,共检出HPV阳性标本58例,阳性率为12.78%;单一、双重、三重和四重基因型感染分别为46例(占79.31%)、4例(占6.90%)、6例(占10.34%)和2例(占3.45%);单纯低危型感染5例(占8.62%),单纯高危型感染46例(占79.31%),低危型与高危型混合感染7例(占12.07%)。共检出17种基因型,包括低危型HPV6、42、43和高危型HPV16、18、31、33、35、45、52、53、56、58、59、66、68、83,低危型以HPV43感染为主,高危型以HPV16、58、52感染为主。结论:健康体检女性进行HPV分型检测对HPV感染和宫颈癌的诊断、治疗具有重要意义。“,”Objective:To explore the prevalence of human papillomavirus(HPV) infection among women receiving health physical examination in Dalian,and provide theoretical basis for screening and preventing cervical cancer.Methods:DNA chip technique of PCR amplification in vitro combined with DNA reverse dot blot was used to qualitatively detect HPV infection among 454 women receiving health physical examination in Dalian.Results:Among 454 women,58 women were found with positive HPV,the positive rate was 12.78%;the proportions of women with single HPV infection,double HPV infection,triple HPV infection,and quadruple HPV infection were 79.31%(46 women),6.90%(4 women),10.34%(6 women),and 3.45%(2 women);the proportions of women with simple low risk HPV infection and simple high low HPV infection were 8.62%(5 women) and 79.31%(46 women),respectively;7 women were found with mixed infection of low risk HPV and high risk HPV,accounting for 12.07%.Seventeen genotypes were screened out totally,including low risk genotypes(HPV6,42,43) and high risk genotypes(HPV16,18,31,33,35,45,52,53,56,58,59,66,68,83);HPV43 infection was the main type among low risk HPV genotypes,and HPV16,58,52 infection were the main types among high risk HPV genotypes.Conclusion:HPV genotyping detection during health physical examination among women has important significance for diagnosis and treatment of HPV infection and cervical cancer.