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目的:研究不同浓度大量、微量、有机元素对丹参毛状根生长量及丹参酮类成分积累的影响。方法:以6,7-V液体培养基为基本培养基,分别将其大量、微量和有机元素的含量设定为缺失(0)、1/8、1/4、1(对照)、4、8倍,取培养相同时间的毛状根测定其增长倍数,利用HPLC法测定二氢丹参酮、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA的含量。结果:大量元素可显著影响丹参毛状根的生长和丹参酮类成分的积累,其中大量元素缺乏时可显著促进丹参酮类成分的积累,但其生长量受到明显抑制,而过量大量元素虽可在一定程度上提高生长量,但丹参酮类成分并无显著增长;微量元素和有机元素的改变对毛状根的生长和丹参酮类成分的积累无显著影响。结论:大量元素是影响丹参毛状根生长和丹参酮类成分积累的主要因素,大量元素缺乏(逆境)可显著促进丹参酮类成分积累,为毛状根培养及不同品质丹参形成机理研究提供了一定的基础。
Objective: To study the effects of different concentrations of trace elements and trace elements on the growth of hairy root and the accumulation of tanshinones in Salvia miltiorrhiza. Methods: The medium of 6,7-V liquid medium was used as basic medium, and the amounts of trace elements, trace elements and organic elements were set as deletion (0), 1/8, 1/4, The hair follicles at the same time were cultured to determine their growth multiples. The contents of dihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone IIA were determined by HPLC. Results: A large number of elements can significantly affect the growth of Salvia miltiorrhiza hairy roots and the accumulation of tanshinones, and the lack of a large number of elements can significantly promote the accumulation of tanshinones, but the growth was significantly inhibited, while excessive large number of elements may be in a certain However, the content of tanshinones did not increase significantly. The changes of trace elements and organic elements had no significant effect on the growth of hairy roots and the accumulation of tanshinones. CONCLUSION: A large number of elements are the main factors that affect the growth of hairy roots and the accumulation of tanshinones in Salvia miltiorrhiza. Lack of macronutrients (Adversity) can significantly promote the accumulation of tanshinones, which provides some evidence for the development of hairy roots and the formation mechanism of Salvia miltiorrhiza basis.