论文部分内容阅读
采用ISSR分子标记对中国水域隶属于不同江豚(Neophocaena phocaenoides)亚种的两个群体即长江群体和渤海群体的遗传多样性进行分析。用19条ISSR引物及3对引物组合对两个群体共36个样品的基因组DNA进行PCR扩增,共得到115个清晰的扩增位点,其中多态性位点48个,多态位点百分率(PPL)为41.71%。POPGENE分析结果表明:两个江豚群体的总体遗传多样性水平相对较低(He=0.1643;Ho=0.2413);长江群体的遗传多样性水平(He=0.1530;Ho=0.2223)稍高于渤海群体(He=0.1402;Ho=0.205 9)。Ne i’s遗传多样性分析结果表明群体结构水平较低而基因流水平较高(Gst=0.104 9;Nm=4.267 6),提示在历史上可能发生过较强的基因交流。我们建议在保护上将两个群体划分为不同的管理单元。
ISSR molecular markers were used to analyze the genetic diversity of two populations in the Chinese waters belonging to the Neophocaena phocaenoides subspecies, the Yangtze River population and the Bohai Sea. Nineteen ISSR primers and three pairs of primers were used to amplify genomic DNA of 36 samples from two populations. A total of 115 clear amplification sites were obtained, including 48 polymorphic loci The percentage (PPL) was 41.71%. The results of POPGENE analysis showed that the overall genetic diversity of the two porpoises was relatively low (He = 0.1643; Ho = 0.2413); the genetic diversity of the Yangtze River population was slightly higher than that of the Bohai Sea population (He = 0.1530; Ho = 0.2223) He = 0.1402; Ho = 0.205 9). Nei’s genetic diversity analysis showed that the population structure was lower and the gene flow was higher (Gst = 0.104 9; Nm = 4.267 6), suggesting that strong gene exchange may have occurred in history. We recommend that the two groups be divided into different management units.