论文部分内容阅读
本研究目的在于分析重度苯中毒患者外周血单个核细胞中T细胞受体删除DNA环 (T cellreceptorexci sionDNAcircle ,TREC)的含量 ,从而了解其初始 (naive)T细胞的含量和胸腺近期输出功能。利用实时定量PCR和TaqMan方法检测 8例重度苯中毒患者和 16例正常人外周血单个核细胞 (PBMNC)中TREC的水平。结果显示正常人中TREC含量为 :6 .6 9± 4 .79 10 0 0PBMNC ;而重度苯中毒患者则为 1.0 3± 0 .4 4 10 0 0PBMNC ,显著低于正常人TREC水平 (P <0 .0 1) ,并持续苯中毒的全过程 ,即使在外周血血象基本恢复正常时 ,TREC水平仍处于较低状态。结论 :重度苯中毒患者胸腺近期输出功能明显低下 ,苯中毒明显损伤T细胞免疫功能。
The objective of this study was to analyze the content of T cell receptor-deleted DNA (TCR) in peripheral blood mononuclear cells from patients with severe benzene poisoning, and to understand the content of naive T cells and the function of the thymus in the short term. The levels of TREC in peripheral blood mononuclear cells (PBMNC) from 8 severe benzene poisoning patients and 16 healthy controls were detected by real-time PCR and TaqMan. The results showed that the TREC content of normal people was: 6.96 ± 4.79 10 0 0 PBMNC, while that of severe benzene poisoning was 1.0 3 ± 0. 4 4 0 0 0 PBMNC, which was significantly lower than that of normal people (P <0 .0 1), and continue the whole process of benzene poisoning, even in the peripheral blood blood returned to normal when the level of TREC is still low. Conclusions: The output function of thymus in severe benzene poisoning patients is obviously low, and benzene poisoning can obviously damage the immune function of T cells.