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目的:探讨川芎嗪(LGT)、维生素C(VitC)联合使用对肝缺血-再灌注损伤(HIRI)时肝细胞能量代谢的影响及其机制。方法:实验兔40只,随机分为肝缺血-再灌注组(A组)和肝缺血-再灌注+ LGT治疗组(B组)、肝缺血-再灌注+VitC治疗组(C组)和肝缺血-再灌注+LGT+VitC治疗组(D组)。在再灌注45min时,分别检测肝组织内三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)含量、总腺苷酸量(TAN)、能荷(EC)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性及一氧化氮代谢产物(NO_2~-/NO_3~-)水平。结果:与A组比较,D组肝组织内ATP、TAN、EC、NO_2~-/NO_3~-含量及SOD活性均明显增高(P＜0．05,P＜0．01),MDA含量显著减少(P＜0．01)。结论:LGT联用VitC可通过降低体内氧自由基水平、提高一氯化氮水平,而改善缺血-再灌注损伤肝脏的能量代谢。
Objective: To investigate the effects of ligustrazine (LGT) and vitamin C (VitC) on the energy metabolism of hepatocytes during hepatic ischemia-reperfusion injury (HIRI) and its mechanism. Methods: Forty experimental rabbits were randomly divided into two groups: ischemia - reperfusion (A) group and ischemia - reperfusion + LGT group (B) ) And liver ischemia-reperfusion + LGT + VitC treatment group (group D). At 45 minutes after reperfusion, the levels of ATP, ADP, AMP, TAN, EC, (MDA), superoxide dismutase (SOD) activity and nitric oxide metabolites (NO 2 - / NO 3 - -) were measured. Results: Compared with group A, the contents of ATP, TAN, EC, NO 2 - / NO 3 - and SOD in group D were significantly increased (P <0.05, P <0.01) (P <0.01). Conclusion: LGT combined with VitC can improve the energy metabolism of liver in rats with ischemia-reperfusion injury by lowering the level of oxygen free radicals in vivo and increasing the level of nitrogen monochloride.