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目的 研究基质金属蛋白酶 -2(MMP2)、基质金属蛋白酶组织抑制剂- 2(TIMP2)在肾透明细胞癌(RCCC)中的表达及意义。 方法 采用免疫组织化学SAB法测定 44例RCCC及20例正常肾组织中MMP2与TIMP2蛋白的表达。44例RCCC中病理分级G1 14例,G2 28例,G3 2例,病理分期pT1 24例,pT2 8例,pT3 12例。 结果 MMP2在RCCC中的阳性表达率为 79. 6% (35 /44),高于正常肾组织的 15% ( 3 /20 ),MMP2表达与RCCC病理分级、分期呈正相关 (r=0. 6316,P<0. 01;r=0.5096,P<0. 01);TIMP2在RCCC中的阳性表达率为 100% (44 /44),高于正常肾组织的 35% (7 /20),但TIMP2表达与RCCC病理分期、分级无相关性(r=0.2404,P>0. 05;r=-0.1236,P>0. 05)。在RCCC中MMP2与TIMP2阳性表达率无相关性 (r=0.2015,P>0. 05 )。 结论 RCCC中MMP2表达与RCCC病理分期、分级呈正相关,提示MMP2免疫活动增高不良预后有关系,而TIMP2的反常表达以及作用值得进一步深入研究。MMP2可以作为判断RCCC预后的分子指标。
Objective To investigate the expression of MMP2 and TIMP2 in renal clear cell carcinoma (RCCC). Methods Immunohistochemical SAB method was used to detect the expression of MMP2 and TIMP2 protein in 44 cases of RCCC and 20 cases of normal renal tissues. There were 14 cases of G1, 28 cases of G2 and 2 cases of G3 in 44 cases of RCCC, 24 cases of pT1, 12 cases of pT2 and 12 cases of pT3. Results The positive expression rate of MMP2 in RCCC was 79.6% (35/44), which was higher than that in normal renal tissues (3/20). The expression of MMP2 was positively correlated with the pathological grade and stage of RCCC (r = 0.6316 , P <0.01; r = 0.5096, P <0.01). The positive expression rate of TIMP2 in RCCC was 100% (44/44), which was higher than that in normal renal tissue (35%, 7/20) There was no correlation between TIMP2 expression and pathological stage and grade of RCCC (r = 0.2404, P> 0.05; r = -0.1236, P> 0.05). There was no correlation between MMP2 and TIMP2 positive rate in RCCC (r = 0.2015, P> 0.05). Conclusion The expression of MMP2 in RCCC is positively correlated with the pathological stage and grading of RCCC, which suggests that MMP2 may have an impaired prognosis. However, the abnormal expression of TIMP2 and its role deserve further study. MMP2 can be used as a molecular indicator to judge the prognosis of RCCC.