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目的:对山海螺药材中紫丁香苷进行薄层鉴别及含量测定。方法:以紫丁香苷为对照品,进行薄层色谱鉴别,用高效液相色谱法测定紫丁香苷的含量。采用Agilent C18(150 mm×4.6 mm,5μm)色谱柱,甲醇-水(20-80)为流动相,检测波长为265 nm,流速0.8 mL/min。结果:薄层色谱中紫丁香苷的斑点清晰;高效液相色谱法精密度、重复性与稳定性良好,紫丁香苷在0.1652~1.652μg范围内有较好的线性关系,平均加样回收率为98.67%,RSD为0.91%。结论:该方法可用于山海螺的鉴别和紫丁香苷的含量测定。
Objective: To identify and determine the content of syringin in mountain conch. Methods: Syringomycin was used as a reference substance to identify by TLC, and the content of syringin was determined by high performance liquid chromatography. An Agilent C18 (150 mm × 4.6 mm, 5 μm) column was used with methanol-water (20-80) as the mobile phase at a detection wavelength of 265 nm and a flow rate of 0.8 mL / min. Results: The spots of syringin in TLC were clear. The precision, repeatability and stability of HPLC were good. The syringin had a good linearity in the range of 0.1652 ~ 1.652μg with the average recovery rate 98.67%, RSD 0.91%. Conclusion: This method can be used for identification of conch and determination of syringin content.