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以长期施用农药和不施用农药条件下的两个拟环纹豹蛛(亚)种群为试验材料,采用Sephacryl S-200层析柱对其群体内的乙酰胆碱酯酶进行了纯化以及活性测定。研究结果显示,Sephacryl S-200纯化乙酰胆碱酯酶的效果理想,纯化后的酶液活力有效分离成分高;两(亚)种群样品纯化后的酶总活力差异显著(P<0.05)。结果表明,受农药长期胁迫影响的种群在较小的地理范围内产生适应性遗传分化(进化)和遗传组成差异,迫使种群对局部环境产生适应性变异,以保持其优势种地位。
Two populations of Leptospira pachyrhizi (sub) population under long-term application of pesticides and non-application of pesticides were used as experimental materials to purify and determine the activity of acetylcholinesterase in their population using Sephacryl S-200 column. The results showed that Sephacryl S-200 was effective in purifying acetylcholinesterase, and the active and effective fractions of the purified enzyme liquid were high. The total enzyme activity of two (sub) population samples was significantly different (P <0.05). The results showed that populations affected by long-term pesticide stress produced adaptive genetic differentiation (evolution) and genetic composition differences in a small geographical range, forcing the population to adapt to the local environment adaptive variation in order to maintain its dominant position.