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目的:环介导等温扩增技术检测人乳头瘤病毒HPV16、18的应用研究。方法:收集宫颈癌行HC-Ⅱ-HPV检测标本高危型的47例,低危型34例。阴性对照20例,分别利用环介导等温扩增(LAMP)方法和PCR方法进行检测,并对检测结果进行比对分析。结果:HC-Ⅱ-HPV筛查的高危组标本中,LAMP检出HPV16和HPV18的阳性率分别是37%和6%,均高于PCR的31%和4%,在HC-Ⅱ-HPV筛查的低危组标本中,LAMP法和PCR法均有高危型别的检出。LAMP法略优于PCR法,二者比较无统计学差异(P>0.05)。结论:LAMP法在HPV高危型16、18的筛查中与PCR法无差异,然而LAMP法是更经济、便捷的HPV16、18亚型筛查方法。
Objective: To study the application of loop-mediated isothermal amplification in the detection of human papilloma virus HPV16,18. Methods: Forty-seven patients with HC-Ⅱ-HPV in high risk type and 34 patients in low risk type were collected. Negative control 20 cases, respectively, using ring-mediated isothermal amplification (LAMP) method and PCR method for detection, and the test results were compared and analyzed. Results: The positive rates of HPV16 and HPV18 in HC-Ⅱ-HPV screening high-risk group were 37% and 6% respectively, which were higher than 31% and 4% Check the low-risk group of specimens, LAMP method and PCR were detected in high-risk type. LAMP method slightly better than PCR method, the two was no significant difference (P> 0.05). Conclusion: The LAMP method has no difference with PCR method in the screening of high-risk HPV types 16 and 18, however, LAMP method is a more economical and convenient screening method for HPV16 and 18 subtypes.