本文旨在了解浙江省大盘山脉鼠类感染巴贝西原虫的流行病学情况。捕获大盘山脉磐安县区域的鼠类共231份,取肝脾标本进行PCR检测。所检测到的PCR阳性结果进行序列测定,并进行分析。从231份鼠肝脾标本中检测发现3例DNA阳性片段,来自针毛鼠和社鼠。核酸序列分析显示与巴贝西原虫DNA片段高度相似。利用PCR方法检测到巴贝西原虫DNA,提示浙江大盘山脉存在鼠类感染巴贝西原虫的状况。 The purpose of this article is to understand the epidemiology of Babesiosis parasitized by rodents in the Great Wall Mountains of Zhejiang Province. A total of 231 rodents were captured in Pan’an County, and liver and spleen samples were collected for PCR detection. The PCR positive results detected were sequenced and analyzed. From 231 samples of rat liver and spleen specimens detected three cases of DNA positive fragments, from the needle hair rat and social mice. Nucleic acid sequence analysis showed a high degree of similarity to the B. berberis DNA fragment. The DNA of Babesiia protozoa was detected by PCR method, which indicated the existence of murine infection of Babesi protozoan in Zhejiang DaPang mountain range.