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采用Westernblot、氚 胸腺嘧啶 ( 3H TdR)和氚 亮氨酸 ( 3H Leu )掺入等技术和方法 ,用血管紧张素Ⅱ(AngⅡ )和血管紧张素 ( 1 7) [Ang ( 1 7) ]刺激大鼠血管平滑肌细胞 (VSMCs) ,观察和分析Ang ( 1 7)对VSMCs增殖及蛋白激酶C (PKC)和胞外调节蛋白激酶 (ERK)表达的影响。Ang ( 1 7)能明显抑制基础和AngⅡ刺激下的VSMCsPKC ζ和ERK1/ 2蛋白表达 (P <0 0 1或P <0 0 5 ) ,减少3H TdR和3H Leu掺入量 (P <0 0 1或P <0 0 5 )。结果提示 ,Ang ( 1 7)对VSMCs增殖有抑制作用 ,这可能与影响PKC ζ和ERK1/ 2蛋白表达有关。
The cells were stimulated with angiotensin Ⅱ (AngⅡ) and angiotensin (Ang) 1 (Ang7) by Western blot, 3H TdR incorporation and 3H Leu incorporation (VSMCs). The effects of Ang (1 7) on the proliferation and the expressions of protein kinase C (PKC) and extracellular regulated protein kinase (ERK) in VSMCs were observed and analyzed. Ang (17) significantly inhibited the expression of PKC ζ and ERK1 / 2 proteins (P <0.01 or P <0 05) and reduced 3H TdR and 3H Leu incorporation (P <0 0) 1 or P <0 0 5). The results suggest that Ang (17) inhibits the proliferation of VSMCs, which may be related to the influence of PKC ζ and ERK1 / 2 protein expression.