论文部分内容阅读
c DNA微阵列和寡核苷酸芯片是常见的合成后点样的 DNA微阵列 ,点样方法主要是通过物理吸附或共价结合的方式将探针固定于载体上。本文总结了近年来国内外文献报道的 c DNA微阵列制备方法 :在多聚赖氨酸包被的玻璃基片表面制备 c DNA微阵列 ;用琼脂糖包被的玻璃基片制备 c DNA微阵列 ;在氨基或醛基修饰的玻璃基片表面制备 c DNA微阵列 ;寡核苷酸芯片的制备方法 :氨基修饰的玻片与 5 `末端带氨基的寡核苷酸探针通过不同的 linker连接 ;硅烷化寡核苷酸直接点样于玻片上制成寡核苷酸微阵列 ;硫代寡核苷酸通过二硫键与巯基修饰的玻片连接 ;水凝胶芯片固定寡核苷酸 ;丙烯酰胺硅烷化的基片与 5′丙烯酰胺修饰的寡核苷酸连接。并展望了基因芯片的应用前景
c DNA microarrays and oligonucleotide microarrays are common post-synthesis spotted DNA microarrays. The spotting method mainly fixes the probe to the carrier through physical adsorption or covalent binding. In this paper, we summarize the preparation methods of c DNA microarrays reported in domestic and foreign literature in recent years: prepare c DNA microarray on the surface of polylysine coated glass substrate; prepare c DNA microarray using agarose coated glass substrate Preparation of c DNA microarray on the surface of amino or aldehyde-modified glass substrate; Preparation of oligonucleotide chip: Amino-modified glass slides are connected with 5 ’terminal amino-containing oligonucleotide probes through different linkers ; Silylated oligonucleotide directly spotted on the slide made of oligonucleotide microarray; thio-oligonucleotide by disulfide bond with the thiol-modified slide connection; hydrogel chip-immobilized oligonucleotide; The acrylamide silylated substrate is attached to a 5 ’acrylamide-modified oligonucleotide. And prospects of the application of gene chips