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用布鲁氏菌属６种布鲁氏菌第１生物型参考菌株、Ｓ１９。和Ｍ５菌苗菌株、ＹｅＯ：９、Ｅ．ｃｏｌｉＯ：１１６和Ｏ：１５７以及士拉伦斯菌分别感染豚鼠每组１０只。从感染后７天开始取血检查，观察１７个月。以布鲁氏菌声波抗原和ＢＳＡｇ作ＥＬＩＳＡ试验，证实所有布鲁氏菌株感染动物中，在观察期内均存在抗声波抗原和抗ＢＳＡｇ的抗体。除Ｂｒ．ｏｖｉｓ和Ｂｒ．ｎｅｏｔｍａｅ感染的动物外（５个月），在其他布鲁氏菌感染动物中，这两种抗体持续一年以上，在１６Ｍ、５４４Ａ和１３０Ｓ感染动物中可持续１７个月以上。Ｅ．ｃｏｌｉ和土拉伦斯菌感染动物只在布鲁氏菌ＳＡＴ出现短期（５～７个月）的低度阳性反应，在ＦＬＩＳＡ试验中均为阴性。ＹｅＯ：９菌感染动物虽在ＳＡＴ和声波抗原ＥＬＩＳＡ中可出现高滴度的阳性反应，且持续时间与ｓ型布鲁氏菌感染一样长，但这些动物缺少抗ＢＳＡｇ抗体因此可用布鲁氏菌ＢＳＡｇＥＬＩＳＡ作布鲁氏菌病的血清学诊断试验。
Six Brucella strains of the first bio-type reference strain of Brucella, S19. And M5 vaccine strains, YeO: 9, E. coliO: 116 and O: 157 and Shigella were respectively infected with 10 guinea pigs. Blood samples were taken from 7 days after infection and observed for 17 months. With Brucella sonicated antigen and BSAg ELISA test, confirmed that all Brucella strains infected animals, there are anti-sonic antigens and anti-BSAg antibody during the observation period. In addition to Br. ovis and Br. (5 months) outside of those infected with neotmae, the two antibodies persisted for more than one year in other animals infected with brucellosis and were sustained in 16M, 544A and 130S infected animals for more than 17 months. E. Low-grade (5-7 months) low-grade positive response to the Brucella SAT was observed in both the E. coli and T. contaminum animals and negative in the FLISA test. Yeo: 9 Infected animals Although there is a high titer of positive reaction in the SAT and sonicated ELISA, and the duration is as long as the infection with brucella s, these animals are deficient in anti-BSAg antibodies and can therefore be treated with Brucella BSAgELISA serological diagnosis of brucellosis test.