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目的观察和评价B ruc ine对移植性肝癌Heps模型荷瘤小鼠的肿瘤抑制作用和生存时间的影响,同时考察B ru-c ine对其免疫系统、造血系统及肝、肾的毒性。方法用ICR♂小鼠接种肝癌Heps瘤株造成移植性肝癌Heps小鼠模型,以B ruc ine对荷瘤小鼠的抑瘤率和生命延长率代表B ruc ine的抗肿瘤活性;以小鼠的体重、免疫器官指数、血细胞指数和肝、肾指数为指标观察B ruc ine对小鼠的毒性及可能的作用机制。结果B ruc ine对移植性肝癌Heps荷瘤小鼠的抑瘤率分别为30.34%(1.61 mg.kg-1)、46.21%(3.23mg.kg-1)和42.07%(6.46 mg.kg-1)。3.23 mg.kg-1(1/20 LD50)是其最佳剂量。但对其生存时间无延长作用。毒性考察实验显示B ruc ine对肝癌Heps小鼠的造血、免疫系统以及肝、肾无明显的毒性。相反B ruc ine还能提高其免疫器官的重量和指数,提高肝癌Heps小鼠的白细胞和血小板数,并能降低小鼠因接种肝癌Heps瘤株而造成的AST、ALT和BUN异常升高。结论B ruc ine能有效抑制移植性肝癌模型荷瘤小鼠体内肿瘤生长,短期对动物的造血、免疫系统以及肝肾没有明显的毒性,相反还能刺激和促进造血系统和免疫系统的功能,恢复小鼠因接种肝癌Heps瘤株而造成的肝肾功能的损伤。通过深入研究B ruc ine有可能发展成为一种新型抗癌药。
Objective To observe and evaluate the effects of B ruc ine on tumor suppression and survival in HepG-bearing mice and to investigate the toxicity of B ru-c ine to the immune system, hematopoietic system and liver and kidney. Methods Hepatocarcinoma Heps mice were inoculated with ICR♂ mice to induce transplanted hepatocarcinoma Heps mouse model. The tumor inhibition rate and life prolongation rate of Brugster on tumor-bearing mice were used to represent the anti-tumor activity of Brucine. Body weight, immune organ index, blood cell index and liver and kidney index as indicators to observe the toxicity of B ruc ine in mice and possible mechanism of action. Results The tumor inhibitory rates of B ruc ine in Hep3 tumor-bearing mice were 30.34% (1.61 mg.kg-1), 46.21% (3.23 mg.kg-1) and 42.07% (6.46 mg.kg-1) ). 3.23 mg.kg-1 (1/20 LD50) is its optimal dose. However, their survival time without extension. Toxicity tests showed that B ruc ine had no obvious toxicity on the hematopoietic, immune system, liver and kidney of Heps mice. In contrast, rupene increases the weight and index of its immune organs, increases the number of leucocytes and platelets in Heps mice, and decreases the abnormally elevated AST, ALT, and BUN in mice that have been inoculated with the HepG2 tumor. Conclusion B ruc ine can effectively inhibit tumor growth in tumor-bearing mice with transplanted hepatocarcinoma. In the short term, it has no obvious toxicity on the hematopoietic, immune system and liver and kidney in animals. On the contrary, B ruc ine can stimulate and promote the function of hematopoietic system and immune system. Mouse liver and kidney function damage due to inoculation of Hepatoma Heps tumor. B ruc ine may develop into a new type of anticancer drug through further research.