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目的探讨顺铂(CP)染毒大鼠睾丸组织病理学和睾丸酶活力的变化。方法选择健康成年雄性Wistar大鼠,随机分为CP 1.0、2.5和5.0 mg/kg组及对照组,连续腹腔注射染毒3 d。染毒结束后,制备PAS(Periodic AcidSchiff)染色病理切片,观察睾丸组织病理变化并行定量组织学分析。分光光度法测定琥珀酸脱氢酶(SDH)、苹果酸脱氢酶(MDH)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、诱导型一氧化氮合酶(i NOS)和Na+-K+-ATPase活力。实时荧光定量PCR检测i NOS和Na+-K+-ATPasemRNA表达水平。结果 CP染毒后大鼠睾丸组织病理学改变主要见于睾丸生精上皮精子发生周期第Ⅱ-Ⅲ期。定量组织学分析发现,在生精上皮第Ⅱ-Ⅲ期CP 2.5和5.0 mg/kg组大鼠睾丸生精小管相对面积和直径变小,管腔相对面积增加(P<0.05)。CP各剂量组生精上皮占生精小管面积百分比和生精小管管腔直径均变小(P<0.05),CP5.0 mg/kg组睾丸间质血管面积增加(P<0.01)。与对照组比较,CP 5.0 mg/kg组精子数减少,精子活力降低(P<0.05)。CP各染毒组SDH活力均低于对照组,而MDH和i NOS活力仅5.0 mg/kg组低于对照组(P<0.05)。CP 5.0 mg/kg组ACP、AKP和Na+-K+-ATPase活力均明显高于对照组(P<0.05)。RT-PCR结果显示,Na+-K+-ATPase和i NOS mRNA表达水平与酶活力改变一致。结论顺铂可能参与改变睾丸细胞能量代谢相关酶活力而致睾丸细胞损伤。
Objective To investigate the changes of testicular histopathology and testicular enzyme activity in cisplatin (CP) -treated rats. Methods Healthy adult male Wistar rats were randomly divided into CP 1.0, 2.5 and 5.0 mg / kg groups and control group. The rats were injected intraperitoneally for 3 days. After the treatment, the pathological sections of PAS (Periodic Acid Schiff) were prepared and histopathological changes of testis were observed and analyzed quantitatively. The contents of SDH, MDH, ACP, AKP, iNOS and Na + were determined by spectrophotometry. -K + -ATPase activity. Real-time fluorescence quantitative PCR was used to detect the expression of iNOS and Na + -K + -ATPase mRNA. Results The histopathological changes of testis in rats after CP exposure were mainly found in stage Ⅱ-Ⅲ of spermatogenic epithelium in testis. Quantitative histological analysis showed that the relative area and diameter of seminiferous tubules of testis in CP 2.5 and 5.0 mg / kg groups in stage II-III of seminiferous epithelium were smaller and the relative area of the lumen was increased (P <0.05). The percentages of seminiferous epithelium and the diameter of seminiferous tubules in CP group were significantly decreased (P <0.05), and the area of testicular interstitium in CP5.0 mg / kg group was increased (P <0.01). Compared with the control group, the number of sperm in CP 5.0 mg / kg group decreased and the sperm motility decreased (P <0.05). The activity of SDH in CP group was lower than that in control group, while the activity of MDH and i NOS in 5.0 mg / kg group was lower than that in control group (P <0.05). The activities of ACP, AKP and Na + -K + -ATPase in CP 5.0 mg / kg group were significantly higher than those in control group (P <0.05). RT-PCR results showed that the expression of Na + -K + -ATPase and iNOS mRNA was consistent with the change of enzyme activity. Conclusions Cisplatin may be involved in the alteration of the activity of enzymes related to energy metabolism in testicular cells, which leads to the damage of testicular cells.