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目的监测乌鲁木齐地区婴幼儿腹泻轮状病毒(RV)感染情况和流行特点。方法 2006年1月至12月,采集门诊或住院≤5岁婴幼儿腹泻样本248例,用酶免法(ELISA)筛查RV,用RT-PCR方法对RV阳性毒株进行G基因型分型。采用SPSS13.0统计软件,数据处理用χ2检验。P<0.05表示统计学差异有统计学意义。结果 248例腹泻样本,检出RV 133例,阳性率为53.6%。RV感染对象主要为24月以内婴幼儿,占94.7%,发病高峰在8~9月份。对125份RV阳性毒株进行G基因型分型,结果显示:G1型(46.4%)为最流行毒株,其次为G3型(17.6%)、G9型(7.2%)和G2型(4.0%),混合感染占14.4%,不能分型占10.4%。结论轮状病毒是乌鲁木齐地区婴幼儿腹泻的主要病原,2006年G1和G3型为主要流行基因型。
Objective To monitor the prevalence and epidemic characteristics of rotavirus infection in infants with diarrhea in Urumqi. Methods From January 2006 to December 2006, 248 infants and young children with out-patient or in-hospital infants diarrhea were collected. 248 samples were screened by enzyme-linked immunosorbent assay (ELISA) and G genotypes were detected by RT-PCR . Using SPSS13.0 statistical software, data processing with χ2 test. P <0.05 means that the statistical difference was statistically significant. Results 248 cases of diarrhea samples were detected RV 133 cases, the positive rate was 53.6%. RV infection mainly for infants and young children within 24 months, accounting for 94.7%, the peak incidence in August to September. The genotypes of G genotypes in 125 RV-positive strains were as follows: G1 (46.4%) was the most prevalent, followed by G3 (17.6%), G9 (7.2%) and G2 (4.0% ), Mixed infection accounted for 14.4%, can not type accounted for 10.4%. Conclusion Rotavirus is the main cause of infant diarrhea in Urumqi region. In 2006, G1 and G3 were the major epidemic genotypes.