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采用反相高效液相色谱法测定人工蛇胆汁中牛磺胆酸、牛磺鹅去氧胆酸及牛磺去氧胆酸 3种主成分的含量。其色谱条件为 :Nova Pak C1 8(4μm )柱 ,流动相 :乙腈 -水 (1∶ 2 ) ,流速 :0 .9m l/ min,检测波长 :2 10 nm。平均加样回收率分别为 :牛磺胆酸 10 0 .5 %、牛磺鹅去氧胆酸 10 2 .2 %及牛磺去氧胆酸 10 0 .9% ,RSD分别为 1.48%、 1.6 0 %及 1.97%。该法快速、灵敏、准确 ,适用于人工蛇胆汁中 3种主要有效成分的同时测定
The contents of main components of taurocholic acid, taurochenodeoxycholic acid and taurodeoxycholic acid in artificial snake bile were determined by reversed-phase high performance liquid chromatography. The chromatographic conditions were: Nova Pak C1 8 (4μm) column, mobile phase: acetonitrile-water (1: 2), flow rate: 0. 9ml/min, detection wavelength: 2 10 nm. The average recovery rates were 100.5% for taurocholic acid, 102.9% for taurochenodeoxycholic acid, and 109% for taurodeoxycholic acid, respectively, with RSDs of 1.48% and 1.6. 0 % and 1.97%. The method is rapid, sensitive, and accurate. It is suitable for simultaneous determination of three main active ingredients in artificial snake bile juice.