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目的探讨Ｋｉ－６７抗原与胃癌病理生物学行为的关系，特别是与远隔脏器转移行为的关系。方法应用ＡＢＣ免疫组化法，检测５６例胃癌手术标本经甲醛固定、石蜡包埋的常规病理切片的Ｋｉ－６７抗原表达，以Ｋｉ－６７抗原表达阳性数评估胃癌组织的增殖活性。结果Ｋｉ－６７抗原表达量与胃癌组织学类型、分化程度、生长方式、胃壁浸润深度以及区域淋巴结转移无明显相关（Ｐ＞０．０５），但与胃癌的肝、卵巢、肾上腺等远隔脏器的转移呈明显正相关（Ｐ＜０．００５）。此外还发现，Ｋｉ－６７抗原表达量与胃癌组织的ＤＮＡ异倍体和突变型ｐ５３基因产物表达呈明显正相关（Ｐ＜０．０５）。结论Ｋｉ－６７抗原作为一个代表增殖活性的肿瘤标志，其过量表达与胃癌的恶性生物学行为，特别是远隔脏器转移行为密切相关，故有可能应用于临床，以估计胃癌的预后和判断远隔脏器转移的危险性。ｐ５３基因可能通过基因突变增加胃癌细胞的增殖活性，使Ｋｉ－６７抗原表达量明显增加，并导致ＤＮＡ异倍体类型的增加。
Objective To investigate the relationship between Ki-67 antigen and the pathological biological behavior of gastric cancer, especially the relationship with distant organ metastasis. Methods ABC immunohistochemical method was used to detect the expression of Ki-67 antigen in routine pathological sections of 56 gastric cancer specimens fixed by formaldehyde and embedded in paraffin. The positive expression of Ki-67 antigen was used to evaluate the proliferation activity of gastric cancer tissues. Results There was no significant correlation between the expression of Ki-67 antigen and the histological type, differentiation, growth pattern, infiltration depth of gastric wall and regional lymph node metastasis (P>0.05). However, the expression of Ki-67 antigen was distant from the liver, ovary and adrenal glands of gastric cancer. The metastasis was significantly positively correlated (P < 0.005). In addition, the expression of Ki-67 antigen was positively correlated with the expression of DNA aneuploid and mutant p53 gene products in gastric cancer tissues (P<0.05). Conclusion Ki-67 antigen is a tumor marker that represents proliferative activity. Overexpression of Ki-67 antigen is closely related to malignant biological behavior of gastric cancer, especially to distant organ metastasis. Therefore, it may be used clinically to estimate the prognosis and judgment of gastric cancer. The risk of distant organ transfer. The p53 gene may increase the proliferation activity of gastric cancer cells through gene mutation, increase the expression of Ki-67 antigen, and lead to the increase of DNA aneuploidy type.