论文部分内容阅读
毛鱼藤（Ｄｅｒｒｉｓ ｅｌｌｉｐｔｉｃａ（Ｒｏｘｂ．） Ｂｅｎｔｈ．）和西非灰毛豆（Ｔｅｐｈｒｏｓｉａ ｖｏｇｅｌｉｉ Ｈｏｏｋ．ｆ．）离体培养物经９：１ＣＨＣｌ３／ＭｅＯＨ溶剂提取，Ｃ－１８柱层析分离后，在室温条件下以６６：３４ＭｅＯＨ／Ｈ２Ｏ或４２：５８ ＣＨ３ＣＮ／Ｈ２Ｏ为流动相，对样品进行反相柱ＨＰＬＣ分析。结果表明，毛鱼藤和西非灰毛豆的愈伤组织均能合成鱼藤酮，但含量相当低，分别为 １７．４ ｕｇ／ｇ，１．８ ｕｇ／ｇ干重。经诱导生根分化的毛鱼藤愈伤组织鱼藤酮含量达１３６．２ ｕｇ／ｇ干重，为愈伤组织含量的７．８倍。西非灰毛豆悬浮培养物的鱼藤酮含量虽比愈伤组织有很大提高，但还不及毛鱼藤愈伤组织含量。结果表明，植物的基因类型在次生代谢上起重要作用，同时离体组织或细胞的分化有助于鱼藤酮的生物合成。
In vitro culture of Derris elliptica (Roxb.) Benth. And Tephrosia vogelii Hook.f. was extracted by 9: 1 CHCl3 / MeOH solvent. After separation by C-18 column chromatography, The sample was subjected to reverse-phase HPLC with 66:34 MeOH / H2O or 42:58 CH3CN / H2O as the mobile phase. The results showed that rotenone could be synthesized from both calluses of both Malus hupehensis and West African gray soybean, but its content was very low, which was 17.4 μg / g and 1.8 μg / g dry weight, respectively. After induced rooting, the content of rotenone in rotten mulberry leaves reached 136.2 ug / g dry weight, which was 7.8 times of the callus content. Although the content of rotenone in West African gray soybean suspension culture is much higher than that of callus, it is still lower than the content of calli. The results showed that plant genotypes play an important role in secondary metabolism, while ex vivo tissue or cell differentiation contributes to the biosynthesis of rotenone.