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目的:为了保证微生物检测质量,了解微生物检测技术人员对肠道致病菌(沙门菌和志贺菌等)分离鉴定技术。方法:应用沙门菌、志贺菌、大肠埃希菌制备定量的混合菌株盲样。统一操作方法,发放统一培养基。结果:参加室间质控的9家单位对高、中、低浓度的三支混合菌盲样检测,特别是中、低浓度的混合菌盲样检测,结果差别较大。结论:盲样中有大量的干扰菌(大肠埃希菌)存在,沙门菌和志贺菌均被大肠埃希菌所掩盖,当微生物检测人员的技能不过硬时,就很难将盲样中低菌量的沙门菌和志贺菌分离出来,影响阳性检出率。因此,应加强微生物检测技术人员基础技能培训,进一步提高技术水平。
Objective: In order to ensure the quality of microbial detection, understand the microbial detection technicians on intestinal pathogens (Salmonella and Shigella, etc.) separation and identification techniques. Methods: Salmonella, Shigella and Escherichia coli were used to prepare quantitative mixed samples. A unified method of operation, issued a unified medium. Results: Nine laboratories participating in room quality control blindly tested three mixed bacteria with high, medium and low concentrations, especially the blind samples with medium and low concentrations of mixed bacteria. The results were quite different. CONCLUSIONS: A large number of interfering bacteria (Escherichia coli) exist in the blind samples. Salmonella and Shigella are both covered by Escherichia coli. When the skills of the microorganism inspectors are not enough, The amount of Salmonella and Shigella isolated, affecting the positive detection rate. Therefore, we should strengthen the basic skills training of microbial detection technicians to further improve the technical level.