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钩体病是由一群致病性钩端螺旋体(以下简称钩体)引起的自然疫源性急性传染病。1989年8~10月份安康地区一些局部地方发生暴发。为了解本地区钩体病流行现状及特点,我们对暴发时的部份患者和健康人群进行抗体检测并对动物进行带菌调查。方法在发病1周和3~4周分别采50名患者静脉血3ml,309名健康人静脉血3ml,用凝溶试验法作血清抗体测定。取150头猪,570只鼠和30只臭鼩鼱肾脏标本,于25℃~28℃培养1周后镜下观察钩体,每周1次,连续观察4次以上,阳性者再进行菌群型鉴定。为防止杂菌污染,在培养基中加入适量五氟脲嘧
Leptospirosis is a naturally transmitted acute infectious disease caused by a group of pathogenic Leptospira (hereinafter referred to as Leptospira). In August and October 1989, some outbreaks occurred in some areas of Ankang. To understand the status and characteristics of the prevalence of leptospirosis in the region, we conducted antibody tests on some patients and healthy people at the time of the outbreak and carried out a survey on the animals. Methods One week and three to four weeks of illness, 50 ml of venous blood and 3 ml of 309 healthy volunteers were collected from 50 patients respectively. Serum antibody was determined by coagulation test. 150 pigs, 570 rats and 30 stinkbug kidney specimens were collected. After 1 week culture at 25 ℃ ~ 28 ℃, the leptospirosis was observed microscopically, and the leptospirosis was observed once a week for more than 4 times. . In order to prevent bacterial contamination, add appropriate amount of pentafluorouracil to the medium