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总结了在进行玉米DNA指纹分析中,通过系统试验筛选SSR核心引物的原理与方法,指出正确的识读与统计PCR扩增带纹是生物技术操作的基本功。讨论和分析了对品种纯度检测、同一性分析和亲子鉴定中存在的概念性错误。简要地叙述了玉米品种DNA指纹分析所涉及的三个不同技术范畴的基本原理。
The principle and method of screening SSR core primers through systematic experiments are summarized in the analysis of DNA fingerprinting in maize. It is pointed out that the correct reading and counting of PCR amplified bands is the basic work of biotechnological operation. Discussed and analyzed the conceptual errors in variety purity testing, identity analysis and paternity testing. Briefly described the basic principles of three different technical areas involved in DNA fingerprinting of maize varieties.