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目的研究银杏叶总黄酮对脑、心缺氧的保护作用。方法小鼠脑、心缺氧模型分别采用断头和挟闭气管术制备，一氧化氮（ＮＯ）含量用ｇｒｅｅｎ法测定，丙二醛（ＭＤＡ）含量用ＴＢＡ法测定。结果银杏叶总黄酮２５、５０、１００ｍｇｋｇ－２显著延长夹闭气管后小鼠心电图时间和断头喘气时间，并呈量效关系，在２５－１００ｍｇｋｇ－１范围内，银杏叶总黄酮显著降低脑、心组织中ＭＤＡ和ＮＯ含量。结论银杏叶总黄酮对脑、心缺氧有显著保护作用，其作用可能与抑制膜脂质过氧化及减少ＮＯ有关。
Objective To study the protective effect of Ginkgo biloba leaf flavonoids on brain and heart hypoxia. Methods Mouse brain and hypoxic models were prepared by decapitation and trephine closed trachea respectively. Nitric oxide (NO) content was measured by green method. Malondialdehyde (MDA) content was measured by TBA method. RESULTS: Ginkgo biloba flavonoids 25, 50, and 100 mg kg-2 significantly prolonged electrocardiogram time and gasping time in mice after tracheal intubation, and showed a dose-effect relationship. Within the range of 25-100 mg kg-1, total flavonoids in Ginkgo biloba leaves significantly reduced the brain level. , MDA and NO content in heart tissue. Conclusion Ginkgo biloba leaf flavonoids have significant protective effect on brain and heart hypoxia, and its effect may be related to inhibition of membrane lipid peroxidation and reduction of NO.