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淀粉分支酶和颗粒结合型淀粉合成酶是木薯淀粉合成过程的关键酶。本研究利用荧光原位杂交技术对木薯淀粉合成相关的淀粉分支酶基因(SBEI)和颗粒结合型淀粉合成酶基因(GBSSⅠ,GBSSⅡ)在染色体上的位置进行了物理定位分析。结果表明:GBSSⅠ和GBSSⅡ基因分别定位于木薯华南6号的第13号染色体的短臂上和第11号染色体的短臂上,信号位点到着丝点的百分距离分别为26.3±0.1和14.0±0.5,信号检出率分别为12.9%和7.9%。而SBEI基因位于第12号染色体的长臂上,信号位点到着丝点的百分距离为76.2±0.2,信号检出率为9.4%。GBSSⅠ、GBSSⅡ和SBEⅠ基因位于不同的染色体上,互为独立基因。本研究揭示了这些基因在染色体上的分布特点和基因间的位置关系以及连锁情况,为分子辅助育种和比较基因组学研究提供理论基础。
Starch branching enzyme and particle-bound starch synthase are the key enzymes in the process of cassava starch synthesis. In this study, fluorescence in situ hybridization was used to physically locate the SBEI and GBSSⅠ, GBSSⅡ genes involved in the synthesis of cassava starch. The results showed that the GBSSⅠand GBSSⅡ genes were located on the short arm of chromosome 13 and the short arm of chromosome 11 in Huaonan 6, respectively. The distances from the signal point to the centromere were 26.3 ± 0.1 and 14.0, respectively ± 0.5, the signal detection rate was 12.9% and 7.9%. The SBEI gene is located on the long arm of chromosome 12, the distance from the signal point to the centromere is 76.2 ± 0.2, and the signal detection rate is 9.4%. GBSS Ⅰ, GBSS Ⅱ and SBE Ⅰ genes are located on different chromosomes, independent of each other. This study revealed the distribution of these genes in chromosomes and the positional relationship between genes as well as the linkage between the situation for molecular assisted breeding and comparative genomics provide a theoretical basis.