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目的采用HPLC法测定小儿热咳口服液中盐酸麻黄碱的含量。方法采用Hypersil BDS C_(18)色谱柱(250 mm×4.6mm,5μm),流动相为乙腈-1%磷酸(3∶97),流速1.0 m L·min~(-1),检测波长206 nm,柱温25℃。结果盐酸麻黄碱对照品的线性范围为12.0~120.0μg·m L~(-1)(r=0.9996),平均回收率为97.7%,RSD=1.9%(n=6)。结论所用方法快速、简便、重复性好,可用于小儿热咳口服液的质量控制。
Objective To determine the content of ephedrine hydrochloride in Pediatric Rehmanniae Oral Liquid by HPLC. Methods The mobile phase consisted of acetonitrile - 1% phosphoric acid (3:97) at a flow rate of 1.0 m L · min -1 on a Hypersil BDS C 18 column (250 mm × 4.6 mm, 5 μm) , Column temperature 25 ℃. Results The linear range of the reference substance of ephedrine hydrochloride was 12.0-120.0 μg · m L -1 (r = 0.9996). The average recovery was 97.7% and RSD was 1.9% (n = 6). Conclusion The method is rapid, simple and reproducible. It can be used for quality control of Pediatric Rectum.