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Laccase was immobilized on the ceramic-chitosan composite support by using glutaraldehyde as the cross-linking reagent. The immobilization conditions and characterization of the immobilized enzyme were investigated. The immobilization of laccase was successfully realized when 3.0 mL of 1.25 mg/mL of laccase at a pH value of 4.0 reacted with 0.15 g of ceramic-chitosan composite support(CCCS) at 4 ℃ for 24 h. The immobilized enzyme exhibited a maximum activity at pH 3.0. The optimal temperatures for immobilized enzyme were 25 ℃ and 50 ℃. The K_m value of immobilized laccase for ABTS was 66.64 μmol/L at a pH value of 3.0 at 25 ℃. Compared with free laccase, the thermal, operating and storage stability of immobilized laccase was improved after the immobilization.
The immobilization of laccase was successfully realized when 3.0 mL of 1.25 mg / mL of laccase at The pH of the reaction mixture at pH 4.0 with 0.15 g of ceramic-chitosan composite support (CCCS) at 4 ° C. for 24 h. The immobilized enzyme showed a maximum activity at pH 3.0. The optimal temperatures for immobilized enzyme were 25 ° C. and 50 ° C. The K_m value of immobilized laccase for ABTS was 66.64 μmol / L at a pH value of 3.0 at 25 ° C. Compared with free laccase, the thermal, operating and storage stability of immobilized laccase was improved after the immobilization.