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目的:探讨贵州省黔南州布依族和苗族宫颈癌及宫颈上皮内瘤变患者HPV感染及型别分布情况,并比较两组患者宫颈癌及宫颈上皮内瘤变患者HPV感染及型别的差异。方法:2007年1月～2011年10月在贵州省黔南布依族苗族自治州中医医院、贵州省瓮安县人民医院、贵州省都匀市人民医院、贵州省贵定县人民医院4家医院组织病理学诊断为CINⅡ、CINⅢ级和宫颈鳞癌的组织标本167例作为研究对象,按民族将其分布依族组87例和苗族组80例,采用美国Digene公司第2代杂交捕获试验检测HPV感染情况,分别比较两组宫颈癌及宫颈上皮内瘤变患者HPV感染及型别的差异。结果:①HPV总感染率:167例宫颈癌及宫颈上皮内瘤变患者中,HPV检出156例,HPV感染率为94.4%。②HPV感染率:布依族组HPV感染率为88.5%77例,其中宫颈癌、CINⅡ和CINⅢ级中HPV感染的阳性率分别为71.4%、96.6%、96.7%;苗族组HPV感染率为98.8%80例,其中宫颈癌、CINⅡ和CINⅢ级中HPV感染的阳性率分别为96.0%、100.0%、100.0%;两组比较,P<0.01。③HPV型别感染率:布依族组宫颈癌、CINⅡ级及CINⅢ级HPV感染以HPV16、HPV58、HPV31和HPV33多见;苗族组宫颈癌、CINⅡ级及CINⅢ级HPV感染以HPV18、HPV33和HPV58多见,两组比较,P<0.01。④HPV型别多重感染率:布依族组单一型别感染53例,占60.9%,多重感染24例,占27.6%。多重感染中最常见的为HPV16合并的感染;苗族组单一型别感染49例,占61.3%,多重感染30例,占37.5%。多重感染中最常见的为HPV18合并的感染,两组比较,P<0.05。结论:布依族宫颈癌及宫颈上皮内瘤变患者HPV感染及型别分布与苗族存在民族差异。
Objective: To investigate the distribution of HPV infection and its genotypes in Buyi and Miao peoples’ cervical cancer and cervical intraepithelial neoplasia in Qiannan Prefecture of Guizhou Province. The differences of HPV infection and genotype between cervical cancer and cervical intraepithelial neoplasia were compared between the two groups . METHODS: From January 2007 to October 2011, four hospitals in Guizhou Qiannan Buyi and Miao Autonomous Prefecture Hospital, Weng’an County People’s Hospital in Guizhou Province, Duyun People’s Hospital in Guizhou Province and Guiding County People’s Hospital in Guizhou Province were recruited. Rigid diagnosis of CIN Ⅱ, CIN Ⅲ level and cervical squamous cell carcinoma tissue samples of 167 cases as the research object, according to the ethnic distribution of the Yi group of 87 cases and Miao group of 80 cases, using the United States Digene company second generation hybridization capture test HPV infection , Were compared between the two groups of cervical cancer and cervical intraepithelial neoplasia in patients with HPV infection and type differences. Results: ① The total HPV infection rate: Among 167 cases of cervical cancer and cervical intraepithelial neoplasia, 156 HPVs were detected and the HPV infection rate was 94.4%. The HPV infection rate in Buyi group was 88.5%. The positive rates of HPV infection in cervical cancer, CINⅡ and CINⅢ were 71.4%, 96.6% and 96.7% respectively. The HPV infection rate in Miao group was 98.8%. Cases, including cervical cancer, CIN Ⅱ and CIN Ⅲ HPV infection in the positive rates were 96.0%, 100.0%, 100.0%; two groups, P <0.01. HPV type infection rate: Buyi group of cervical cancer, CIN Ⅱ and CIN Ⅲ HPV infection HPV16, HPV58, HPV31 and HPV33 more common; Miao cervical cancer, CIN Ⅱ and CIN Ⅲ HPV infection with HPV18, HPV33 and HPV58 more common , P <0.01 between the two groups. ④PVV type multiple infection rate: Buyi group single type infection in 53 cases, accounting for 60.9%, multiple infections in 24 cases, accounting for 27.6%. In multiple infection, the most common infection was HPV16. In Miao group, 49 cases were single type infection (61.3%) and multiple infections (30.5%), accounting for 37.5%. The most common infection in multiple infections was HPV18 co-infection, P <0.05 for both groups. Conclusion: HPV infection and genotype distribution in Buyi cervical cancer and cervical intraepithelial neoplasia are different from those in Miao ethnic group.