论文部分内容阅读
1.在木瓜蛋白酶作用下,N-酰基-DL-丝氨酸与苯胺作用合成了N-异戊酰-、N-正己酰-、N-苯甲酰-及N-苯甲氧羰酰-L-丝氨酸酰苯胺。 2.脂肪族取代基,正己酰-,取代的丝氨酸酰苯胺不对称合成反应的最适pH为4.9～5.1;芳香族取代基,苯甲氧羰酰-,取代的丝氨酸酰苯胺不对称合成反应的最适pH为5.0～5.2。 3.比较了四种酰基-丝氨酸的酰苯胺合成的速度。N-苯甲氧羰酰-L-丝氨酸酰苯胺最快;N-苯甲酰-,N-正己酰-其次;N-异戊酰-最慢。 4.在最适pH,酶浓度自1.31毫克蛋白N/毫升反应液提高到2.27毫克蛋白N/毫升反应液时,N-苯甲氧羰酰-L-丝氨酸酰苯胺的产率,在24小时自76.43％提高到100％。 5.在pH=5.0～5.2,柠檬酸缓冲液浓度自0.2M提高到1.0M时,N-苯甲氧羰酰-L-丝氨酸酰苯胺的产率,在12小时提高了1％。 6.选定了苯甲氧羰酰基为取代基;反应最适pH=5.0～5.2;酶浓度为2.2毫克蛋白N/毫升反应液;缓冲液浓度为0.2M;作为丝氨酸酰苯胺不对称合成的最适条件,在此条件下进行了实际析解,得到中间产物N-苯甲氧羰酰-L-丝氨酸酰苯胺,与此同时,还得到了N-苯甲酰-、N-正己酰-、N-异戊酰-L-丝氨酸酰苯胺。 7.水解N-苯甲氧羰酰-、N-苯甲酰-、N-正己酰-、N-异戊酰-L-丝氨酸酰苯胺,得到了L-丝氨酸,水解N-苯甲氧羰酰-D-线氨酸,得到了D-丝氨酸,两个旋
1. In the papain under the action of N-acyl-DL-serine and aniline synthesized N-isovaleryl-, N-n-hexanoyl-, N-benzoyl- and N-benzyloxycarbonyl- L- Serine anilide. 2. The optimum pH for the asymmetric synthesis of aliphatic serine, aniline and substituted hexanoyl anilines is 4.9-5.1. Asymmetric synthesis of aromatic substituents, benzyloxycarbonyl-, substituted serine anilides The optimum pH is 5.0 ~ 5.2. The rate of the synthesis of four acyl-serine anilides was compared. N-benzyloxycarbonyl-L-serine anilide fastest; N-benzoyl-, N-n-hexanoyl - followed; N-isovalerate the slowest. 4. Yields of N-benzyloxycarbonyl-L-serine anilide at the optimum pH and enzyme concentration from 1.31 mg protein N / ml reaction to 2.27 mg protein N / ml reaction over 24 hours From 76.43% to 100%. 5. The yield of N-benzyloxycarbonyl-L-serine anilide increased by 1% in 12 hours when the citrate buffer concentration was increased from 0.2M to 1.0M at pH = 5.0-5.2. The optimal reaction pH is 5.0 ~ 5.2; the concentration of enzyme is 2.2 mg protein N / ml reaction solution; the concentration of buffer is 0.2 M; As asymmetric synthesis of serine anilide The optimum conditions, under the conditions of the actual analysis, to give the intermediate product N-benzyloxycarbonyl-L-serine anilide, at the same time, have also been N-benzoyl-, N- , N-isovaleryl-L-serine anilide. 7. Hydrolysis of N-benzyloxycarbonyl-, N-benzoyl-, N-n-hexanoyl-, N-isovaleryl-L-serine anilide to give L-serine, hydrolysis of N-benzyloxycarbonyl The acyl-D-lysine gave D-serine in two spins