论文部分内容阅读
作者用8只无感染的Wistar鼠,于硫喷妥钠麻醉下,经腹侧切口,找到左咽鼓管,用凝固针造成实验性阻塞,4只于2周、另4只于4周后断头,甲醛固定后,打开听泡,将其分为内(鼓岬、咽鼓管口与龛)、外(鼓膜)两半,分别进行有关的组织学检查并作杯状细胞计数。右耳作为对照。结果:正常中耳粘膜厚为150μm,包括上皮、基底膜、固有层及少量血管,看不到腺体;鼓膜与听泡均无杯状细胞;咽鼓管开口外侧面的杯状细胞仅4/视野,而其内侧面为10/视野;具假复层柱状上皮的圆窗龛处,杯状细胞略多。
The authors used eight uninfected Wistar rats, anesthetized with thiopental, ventral incision to find the left eustachian tube, coagulation needle caused by experimental obstruction, 4 in 2 weeks, the other 4 after 4 weeks After decapitation and formaldehyde fixation, open the listening bubble and divide it into inner (promontory, eustachian tube orifice and niche) and outer (tympanic membrane) halves, and conduct histological examination and goblet cell counting respectively. Right ear as a control. Results: The normal middle ear mucosa thickness was 150μm, including the epithelium, basilar membrane, lamina propria and few blood vessels, glands can not be seen; tympanic membrane and listening bubble were no goblet cells; goblet cells on the outer surface of the goblet cells only 4 / Field of vision, while the medial side of 10 / field of vision; with pseudostratified columnar epithelium round window niche, goblet slightly larger.