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1980年12月,西北地区黄斑星天牛科研协作组的同志们,在甘肃省天水市四十里铺大队发现一具该种幼虫僵尸,交我们带回。1981年,我们从事了三项工作:1.僵菌的分离、培养和提纯;2.菌种鉴定;3.对黄斑星天牛幼虫寄生活性的测定。1982年,我们又从事了三项工作:1.生产成品;2.野外定性和定量试验;3.毒力测定。现摘要于下。一、僵菌分离、培养及提纯根据柯克氏假定(Koch,Sopostula-tes),将天牛幼虫僵体组织分离,培养,提纯,再接种,再分离,反复三次。结果看出:所分离培养的菌落颜色,形态和接种后寄主发病过程及症状均一致。从而可得出黄斑星天牛僵体传染病是病原僵菌所致。二、菌种鉴定经镜下鉴定,黄斑星天牛幼虫的致病病原菌是球孢白僵菌 Beauveria bassiana
In December 1980, comrades of the Collaboration Research Group of Astragalus stellareas in Northwest China discovered a zombie of the larva in the Shiliuli shop in Tianshui, Gansu, and handed us back. In 1981, we engaged in three tasks: 1. Isolation, cultivation and purification of Stibnites; 2. Identification of species; 3. Determination of parasitic activity on the larvae of Aspergillus f. In 1982, we also engaged in three tasks: 1. Production of finished products; 2. Field qualitative and quantitative tests; 3. Toxicity determination. The summary is below. Isolation, Culture and Purification of Stibcuin According to Kirk’s hypothesis (Koch, Sopostula-tes), the sessile bullous larvae were isolated, cultured, purified, re-inoculated, and then separated again three times. The results showed that: the colonies isolated culture color, morphology and host disease after inoculation process and symptoms are the same. It can be derived from Astragalus stellareformis is caused by pathogenic actinomycetes. Second, identification of strains identified by microscopy, the pathogenic bacteria of the larvae of the disease is Beauveria bassiana Beauveria bassiana