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目的研究熊果酸(UA)对人乳腺癌SK-BR-3细胞COX-2、Bax、Bcl-2 mRNA的表达的影响,结合人体乳腺癌组织凋亡相关蛋白表达检测,探讨其作用机制,为UA的临床应用提供实验依据。方法 RT-PCR技术检测SK-BR-3细胞中COX-2、Bax、Bcl-2 mRNA的表达;免疫组织化学技术检测雌激素受体(ER)(-),人表皮生长因子受体-2(HER-2)(+)的人乳腺癌组织中COX-2、Bax、Bcl-2蛋白的表达。结果 UA作用48 h后能使乳腺癌SK-BR-3细胞中COX-2、Bcl-2 mRNA表达明显减少(P<0.05),使Bcl-2/Bax比值减小(P<0.05),而对Bax mRNA的表达未见明显影响;COX-2、Bcl-2、Bax蛋白在ER(-),HER-2(+)的乳腺癌组织中的表达阳性率分别为86.67%(26/30),63.33%(19/30),56.67%(17/30),COX-2与Bax表达呈明显负相关(P<0.05),与Bcl-2未见明显相关性。结论 UA可抑制COX-2、Bcl-2 mRNA表达,使Bcl-2/Bax减小;ER(-),HER-2(+)的人乳腺癌组织中COX-2中阳性表达率较高,COX-2与Bax表达呈明显负相关,与Bcl-2表达未见明显相关性。
Objective To investigate the effect of ursolic acid (UA) on the expression of COX-2, Bax and Bcl-2 mRNA in human breast cancer SK-BR-3 cells and to explore the mechanism of UA on the expression of apoptosis related proteins in human breast cancer. Provide experimental evidence for the clinical application of UA. Methods The expressions of COX-2, Bax and Bcl-2 mRNA in SK-BR-3 cells were detected by RT-PCR. The expressions of estrogen receptor (ER), human epidermal growth factor receptor-2 The expression of COX-2, Bax, Bcl-2 protein in human breast cancer tissue with HER-2 (+). Results After UA treatment for 48 h, the expression of COX-2 and Bcl-2 mRNA in breast cancer SK-BR-3 cells was significantly decreased (P <0.05), and the ratio of Bcl-2 / Bax was decreased The positive rate of COX-2, Bcl-2 and Bax expression in breast cancer tissues of ER (-) and HER-2 (+) tissues was 86.67% (26/30) , 63.33% (19/30), 56.67% (17/30) respectively. The expression of COX-2 was negatively correlated with Bax expression (P <0.05), but not with Bcl-2. Conclusions UA can inhibit the expression of COX-2 and Bcl-2 mRNA and decrease the expression of Bcl-2 / Bax. The positive rate of COX-2 in ER (-) and HER- COX-2 and Bax expression was negatively correlated, but no significant correlation with Bcl-2 expression.