粪类圆线虫是一种重要的人类线虫病病原体,其在同一宿主体内复制和再感染的独特性给予它明显区别于其他寄生蠕虫的病理学重要性。本文首次报道了编码粪类圆线虫天冬氨酸蛋白酶的基因序列,这项研究将有助于研究者对粪类圆线虫的理解。 作者利用天冬氨酸蛋白酶活跃位点Asp32残基高度保守区域互补的简并寡核苷酸作引物,粪类圆线虫一个杆状幼虫cDNA文库和二个丝状幼虫cDNA文库为模板,经过同源PCR,扩增产物亚克隆,文库筛选,阳性克隆核苷酸测序分析得到一个编码粪类圆 Staphylococcus aureus is an important pathogen of human nematode and the uniqueness of its replication and re-infection in the same host gives it a clear distinction from the pathological importance of other parasitic worms. This paper reports for the first time the gene sequence encoding the stool nematode aspartic protease, which will help researchers understand Stool worms. The authors use degenerate oligonucleotides complementary to the highly conserved Asp32 residues of the aspartic protease active site as primers, a rod-shaped larval cDNA library of C. elegans and two filamentous larvae cDNA libraries as templates, Source PCR, Subcloning of Amplification Products, Library Screening, Positive Clone Nucleotide Sequencing Analysis A cDNA encoding a dung circle